insulin transferrin selenium (Thermo Fisher)

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Thermo Fisher insulin transferrin selenium
Insulin Transferrin Selenium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/insulin+transferrin+selenium/Transferrin/pmc13099361-378-21-23
Average 99 stars, based on 1 article reviews

insulin transferrin selenium - by Bioz Stars, 2026-11

99/100 stars

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Modification:

Article Title: Novel polymeric fluoropyrimidine CF10 demonstrates superior therapeutic index and survival advantage in patient-derived models of 5-fluorouracil-refractory colorectal cancer
Article Snippet: .. Cells were maintained in Iscove’s Modified Dulbecco’s Medium (IMDM; Gibco #12440-053) supplemented with 20% fetal bovine serum (FBS; heat-inactivated), 1% insulin-transferrin-selenium (ITS; Gibco #41400-045), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37°C, 5% CO2 (normoxia). .. Where indicated, cultures were adapted and maintained in physioxia (TX-CC-096h2, TX-CC-286, and TX-CC-199) using a tri-gas incubator, Whitley H35 Hypoxystation (Don Whitley Scientific Ltd., Bingley, UK), at 5% O2 (unless otherwise noted in figure legends).

Article Title: Intraovarian injection of securinine stimulates the growth of small ovarian follicles by reducing stromal suppression.
Article Snippet: .. Ovaries were isolated and cultured on tissue culture plate inserts (MCEP24H48, Millipore, Burlington, MA, USA) in 24- well culture plates containing 500 μL of Dulbecco’s modified Eagle’s medium/nutrient mixture F-12 (DMEM/F-12; 1:1, v/v; Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 1% insulin–transferrin–selenium (41400–045, Invitrogen, Waltham, MA, USA) and 3 mg/mL bovine serum albumin (BSA, A1933, Sigma, St. Louis, MO, USA) at 37°C in an atmosphere of 5% CO2. ..

Suspension:

Article Title: Establishment and characterization of a novel HBV-integrated hepatic sarcomatoid carcinoma cell line.
Article Snippet: Hepatic sarcomatoid carcinoma (HSC) is a rare and highly aggressive liver malignancy with an extremely poor prognosis.. The lack of well-validated cellular models has severely limited mechanistic and translational research on this disease.. In this study, we established and comprehensively characterized a novel HSC cell line, designated PUMC-LICA3.

Cell Culture:

Article Title: Treatment of diuretic resistant heart failure patients having at least one copy of the TMPRSS6 rs855791 allele
Article Snippet: Spironolactone (Selleckchem, Cat#54054) was added at the final concentration of 1 μM or 100 μM 10 min before the addition of IL-6 (Sigma-Aldrich, Cat# SRP3096-20UG) at the final concentration of 10 ng/mL, 100 ng/mL, or 500 ng/mL. .. Methods The mouse M1 CCD cell line (American Type Culture Collection (ATCC), Cat# CRL-2038) was maintained the cell culture medium containing equal amount of DMEM (Sigma-Aldrich, Cat#D6046) and Han F12 (Sigma-Aldrich, Cat#11765-047), supplemented with 5% Fetal Bovine Serum (FBS), 1% Penicillin-Streptomycin (Thermo Fisher Scientific, Cat#15140-122), 1% Insulin-Transferrin-Selenium (Thermo Fisher Scientific, Cat#51500-056) and 100 nM Dexamethasone (Sigma-Aldrich, Cat# D4902-100MG). ..

Article Title: Glabridin improves cardiac remodeling after myocardial infarction by activating PPARγ to regulate the ubiquitination and degradation of EGFR.
Article Snippet: The fibroblasts were cultured in high-glucose DMEM (HyClone, USA) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. .. After 24 hours of culture, the medium was replaced with DMEM containing 1% insulin-transferrin-selenium (Invitrogen, USA) and cultured for an additional 24 hours. .. To induce myocardial fibrosis and evaluate the therapeutic effect, fibroblasts were stimulated with 10 ng/ml TGF-β1 [24] (PeproTech, USA) and Glabridin [25] (HY-N0393, MCE) for 24 hours respectively.

Isolation:

Over Expression:

Article Title: CAS9 fusion proteins, RNA-guided gene editing, gene regulation compositions, and methods comprising the same
Article Snippet: To assess dystrophin protein expression, immortalized myoblasts were differentiated into myofibers by replacing the growth medium with DMEM supplemented with 100 insulin-transferrin-selenium (Invitrogen) and 1% antibiotic/antimycotic (Invitrogen) for 4-7 days, such as 6 or 7 days. .. Fibroblasts were transdifferentiated into myoblasts by inducing MyoD overexpression and incubating the cells in DMEM supplemented with 1% insulin-transferrin-selenium (Invitrogen), 1% antibiotic/antimycotic (Invitrogen) and 3 g/mL doxycycline for 15 days. .. Cryosections were incubated overnight at 4° C. with the following primary antibodies that are specific to human epitopes only: anti-spectrin (1:20, Leica NCL-SPEC1) or anti-dystrophin (1:2, Leica NCL-DYS3).

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